2008. Kwang-il Lim and David V. Schaffer*. "Library selection approaches to engineering enhanced retroviral and lentiviral vectors" / Computational Chemistry & High Throughput Screening

February 1, 2008

 

Retroviral and lentiviral based gene delivery vectors have been used in numerous pre-clinical studies and clinical trials due to their advantages, including stable and prolonged expression of therapeutic transgenes and minimal immune responses against the vector. Despite such advantages, however, retroviral vectors also have several limitations for gene therapy applications. For example, they can suffer from a lack of efficient or targeted gene delivery to key cell types. In addition, retroviral vector stability can be compromised by their envelope proteins. This review briefly describes how such limitations have been overcome by recently developed library selection approaches that borrow a lesson from nature: the ability of evolution to generate biomolecules with novel function. These library selection approaches are based on the construction of retroviral libraries where the sequences encoding natural viral components are partially randomized using a variety of methods in order to generate diverse libraries that can be selected to create improved or novel functions. These high throughput, library-based approaches provide a strong complement to rational engineering of viral components for the rapid development of efficient and safe retroviral and lentiviral vector systems for gene therapy.

 

 

 

PMID: 18336204

 

Share on Facebook
Share on Twitter
Please reload

Recent Posts
Please reload

© 2023 by Kathy Schulders. Proudly created with Wix.com  

  • Grey Twitter Icon